There is little public awareness on the anaesthesiology profession among the Nigerian population. This study aims to determine the level of anaesthesiology profession awareness among the population of tertiary school students, academic and non-academic staff members who were attending a South-western Nigerian zonal conference. Methods: Data obtained from a cross-section of 2,748 students, academic and non-academic staff members of various tertiary institutions in South-western Nigeria were used in this study. The study tool was a self-administered anonymous questionnaire which obtained information on the participants’ demographic profile and knowledge of the anaesthesiology profession. Data analysis was done using SPSS version 16